Alfalfa Extract Powder 10:1, 20:1, 50:1 TLC

Alfalfa Extract Powder 10:1, 20:1, 50:1 TLC

Alfalfa extract is a plant extract prepared from the whole plant of Medicago sativa L., a leguminous plant. It mainly contains active ingredients such as flavonoids, triterpenoids, alkaloids, coumarins, proteins, and polysaccharides. This extract has biological activities such as antibacterial, antioxidant, immune regulation, and cholesterol lowering. As a food grade raw material, it is widely used in the fields of food additives, health ingredients, and pharmaceuticals, and quality testing is carried out using thin-layer chromatography (TLC).

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Alfalfa Extract Powder 10:1, 20:1, 50:1 TLC
Botanical source: Medicago sativa L.
Part used: Whole herb
Specification: 10:1, 20:1, 50:1 TLC
Extraction solvents: Water
Appearance: Brownish fine powder
Particle size: 95% pass 80 mesh size
Main ingredients: Alfalfa extract mainly contains saponins, flavonoids (such as alfalfa extract), isoflavones, and various vitamins. Its saponins help regulate blood lipids, flavonoids have antioxidant effects, and isoflavones exhibit phytoestrogenic activity. In addition, it also contains components such as coumarin and plant sterols, which together support its traditional applications in antioxidant and metabolic regulation.
Storage conditionsStore at room temperature in a sealed manner, away from light, and in a ventilated, cool, and dry environment.
Shelf life 24 months from the production date

Alfalfa Extract Powder Production Flowchart
Alfalfa raw materials -Coarse powder(40 mesh) -Low temperature water extraction – 1st Reflux Extraction(10 times water,2 Hrs) – 2nd Reflux Extraction8 times water,1.5 Hrs) – 3rd Reflux Extraction(6 times water,1 Hrs) – Extraction Solution-combine&Filtrate-Concentrate-Extractum-spray drying – screening – packaging – detection of physical and chemical indicators warehousing

Specification Sheet of Alfalfa Extract Powder
Product name: Alfalfa Extract
Specification: 10:1 TLC
Part used: Whole herb of Medicago sativa L.
Solvent used: Water
Process: Raw materials crushed, extracted, concentrated and spray-dried to powder
Non GMO according to regulation (EC) 1829/2003 and 1830/2003 or United States requirements. Non allergen according to Directive 2007/68 amending Annex IIIa to Directive 2000/13/EC and US Food allergen labelling and consumer protection act 2004.
Heavy Metals:      
Lead: NMT 3ppm Cadmium: NMT 1ppm
Arsenic: NMT 2ppm Mercury: NMT 1ppm
Residual solvents: Comply to USP
Pesticides residues: Conform to Regulation USP<561>
Microbiology:      
Total plate count: 10000cfu/g Max Yeasts and molds: 1000cfu/g Max
E.coli: Not detected in (g)10 Salmonella spp.: Not detected in (g)25
Staphylococcus aureus: Not detected in (g)10 Clostridium spp.: Not Present in 0.1 g of food
Organoleptic quality Method Specifications
Aspect: Visual : ( CQ-MO-148) Powder
Color: Visual : ( CQ-MO-148) Brownish yellow
Flavor: Sensory: (CQ-MO-148) Characteristic
Analytical quality Method Specifications
Identification: TLC Conform
Loss on drying: USP <731> < 10%
Bulk density: USP <616> Method I 40 – 60 g/100mL
Particle size: Analytical sieving || USP <786> 100% through 80meshes
Packaging suitable for foodstuff.

Extended Reading

Chemical Components and Modern Pharmacological Effects of Alfalfa (Medicago sativa)

Key Chemical Components:
Alfalfa contains a diverse array of bioactive compounds, including saponins (e.g., medicagenic acid), flavonoids (tricin, apigenin), coumarins (coumestrol), isoflavones (formononetin), alkaloids (L-canavanine), vitamins (A, K, C), minerals, and dietary fiber.

Modern Pharmacological Effects:

  1. Hypolipidemic & Cardioprotective: Saponins and fibers reduce cholesterol absorption and improve lipid profiles.
  2. Antioxidant: Flavonoids and phenolics neutralize free radicals, mitigating oxidative stress.
  3. Phytoestrogenic Activity: Coumestrol and isoflavones exert estrogen-like effects, potentially aiding menopausal symptoms and bone health.
  4. Anti-inflammatory: Modulates inflammatory pathways (e.g., NF-κB) and cytokine production.
  5. Hepatoprotective: Attenuates liver injury via antioxidant and anti-inflammatory mechanisms.
  6. Antidiabetic: Improves insulin sensitivity and glycemic control, partly through fiber and antioxidant effects.
  7. Diuretic & Detoxifying: Promotes renal excretion of toxins, supported by historical use.

References:

  1. Bora, K. S., & Sharma, A. (2011). Phytochemical and pharmacological potential of Medicago sativa: A review. Pharmaceutical Biology.
  2. Gray, A. M., & Flatt, P. R. (1997). Pancreatic and extra-pancreatic effects of the traditional anti-diabetic plant, Medicago sativaThe Journal of Nutrition.
  3. Bouabdelli, S., et al. (2023). Medicago sativa: A comprehensive review on its phytochemical and pharmacological profile. South African Journal of Botany.
  4. Święciło, M., & Ziaja-Sołtys, M. (2022). Biologically active compounds in alfalfa. Biomolecules.

 Note: This summary is for informational purposes. It may interact with medications and is contraindicated in certain conditions. Consult a healthcare professional before therapeutic use, particularly regarding its estrogenic activity.

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