Olive Leaf Extract Powder 10:1, 20:1, 50:1 TLC, Hydroxytyrosol 10%, 20%, 25% HPLC，Oleuropein 6%，12%，20%，40% HPLC
【Botanical source】: Olea europaea L.
【Part used】: Leaves
【Specification】: 10:1, 20:1, 50:1 TLC, Hydroxytyrosol 10%, 20%, 25% HPLC，Oleuropein 6%，12%，20%，40% HPLC
【Extraction solvents】: Water
【Appearance】: Brownish fine powder
【Particle size】: 95% pass 80 mesh size
【Main ingredients】: The main components of olive leaves are potent polyphenolic substances, especially rich in oleuropein, and contain hydroxytyrosol, flavonoids (such as luteolin), and caffeoylquinic acid. The concentration of its active ingredients is usually higher than that of the fruit, and the core lies in its strong antioxidant, antibacterial, and anti-inflammatory biological activities.
【Storage conditions】：Store at room temperature in a sealed manner, away from light, and in a ventilated, cool, and dry environment.
【Shelf life】： 24 months from the production date

Olive Leaf Extract Powder Production Flowchart
Olive leaf raw materials -Coarse powder(40 mesh) -Low temperature water extraction – 1^st Reflux Extraction(10 times water,2 Hrs) – 2^nd Reflux Extraction8 times water,1.5 Hrs) – 3rd Reflux Extraction(6 times water,1 Hrs) – Extraction Solution-combine&Filtrate-Concentrate-Extractum-spray drying – screening – packaging – detection of physical and chemical indicators – warehousing

Specification Sheet of Olive Leaf Extract Powder

Extended Reading

Chemical Components and Research Highlights on Olive Leaf Extract, with Focus on Hydroxytyrosol and Oleuropein

Olive leaf extract (OLE) is a concentrated source of bioactive compounds, distinct from olive oil or fruit extracts, with its unique profile centered on the secoiridoid oleuropein and its primary metabolite hydroxytyrosol. Modern research focuses on their synergistic and individual mechanisms of action.

Key Chemical Components of OLE:

• Oleuropein: The dominant secoiridoid glycoside, precursor to many active metabolites.
• Hydroxytyrosol: A potent simple phenol formed via hydrolysis of oleuropein; a key marker for extract standardization.
• Ligstroside: A related secoiridoid with similar bioactivity.
• Flavonoids: Luteolin, apigenin, and their glycosides.
• Phenolic Acids: Caffeic, vanillic, and verbascoside.
• Triterpenes: Oleanolic acid, maslinic acid.

Summary of Key Research Findings:

1. Cardiovascular & Metabolic Health

• Mechanism: OLE improves endothelial function (↑NO bioavailability), reduces LDL oxidation, and inhibits angiotensin-converting enzyme (ACE), contributing to blood pressure regulation.
• Clinical Evidence: Human trials show significant reductions in systolic/diastolic blood pressure and improved lipid profiles in pre-hypertensive subjects.

2. Antioxidant & Anti-inflammatory Actions

• Hydroxytyrosol: Recognized as one of the most potent natural antioxidants (ORAC assay), efficiently scavenges peroxynitrite and protects mitochondrial function.
• Oleuropein: Inhibits NF-κB and MAPK pathways, reducing pro-inflammatory cytokines (TNF-α, IL-6) and enzymes (COX-2).

3. Antimicrobial & Antiviral Properties

• Broad-Spectrum Activity: Demonstrated efficacy against bacteria (including H. pylori, MRSA), fungi (e.g., Candida spp.), and viruses (influenza, HIV, SARS-CoV-2 in vitro) via membrane disruption and viral entry/replication inhibition.

4. Neuroprotection

• Both compounds cross the blood-brain barrier, reduce oxidative stress and neuroinflammation in models of Alzheimer’s (↓Aβ aggregation, tau phosphorylation) and Parkinson’s disease (protects dopaminergic neurons).

5. Antidiabetic Effects

• Enhances insulin sensitivity via AMPK activation and GLUT4 translocation; inhibits advanced glycation end-product (AGE) formation and α-glucosidase activity.

6. Anticancer Potential (Preclinical)

• Induces apoptosis and cell cycle arrest in various cancer lines (breast, colon, prostate) through mitochondrial dysfunction and ROS-mediated pathways.

Current Research Gaps & Directions:

• Standardization of extracts (ratio of oleuropein to hydroxytyrosol).
• Long-term safety and pharmacokinetics in humans.
• Synergistic effects with other polyphenols.

References:

1. El, S. N., & Karakaya, S. (2009). Olive tree (Olea europaea) leaves: potential beneficial effects on human health. Nutrition Reviews.
2. Omar, S. H. (2010). Oleuropein in olive and its pharmacological effects. Scientia Pharmaceutica.
3. Robles-Almazán, M., et al. (2018). Hydroxytyrosol: Bioavailability, toxicity, and clinical applications. Food Research International.
4. Lockyer, S., et al. (2017). Secoiridoids delivered as olive leaf extract induce acute improvements in human vascular function and reduction of an inflammatory cytokine: a randomised, double-blind, placebo-controlled, cross-over trial. British Journal of Nutrition.
5. F. Visioli, et al. (2021). Olive oil polyphenols: current knowledge and challenges in human health. European Journal of Lipid Science and Technology.

Note: This summary is for informational purposes. It may interact with medications and is contraindicated in certain conditions. Consult a healthcare professional before therapeutic use, particularly regarding its estrogenic activity.